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Product Data
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Species
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Human
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Expression Host
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E. coli
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Expression cDNA Clone or AA Sequence
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Protein Sequence (show)
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Tag
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Tag Free
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Predicted MW
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17.5 kDa
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Concentration
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>0.1 µg/µL as determined by microplate BCA method
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Purity
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>95% as determined by SDS-PAGE and Coomassie blue staining
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Buffer
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Lyophilized from a sterile solution containing 20 mM PB, pH 7.2
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Note
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For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
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Storage
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Store at -80°C.
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Stability
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Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
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Bioactivity
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Determined by the cytolysis of L929 cells in the presence of Actinomycin D. ED50 for this effect is typically 0.19-0.27 ng/ml.
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Endotoxin
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< 0.1 EU per 1 µg of the protein by the LAL
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